Coding

Part:BBa_K129005:Design

Designed by: Burak YILMAZ   Group: iGEM08_METU_Turkey   (2008-11-08)


LamB_CP-metal binding domain in LamB-153 residue


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1325
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1326
    Illegal PstI site found at 1340
    Illegal NotI site found at 7
    Illegal NotI site found at 1333
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1326
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1326
    Illegal PstI site found at 1340
    Illegal AgeI site found at 98
    Illegal AgeI site found at 1202
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To make HP_LamB GATCCAGCTGGTCATCATCCACACGGTGCT is inserted in LamB protein at 153


Source

GATCCAGCAGGCTGCGGTTGTCCATGCGGTTGTGGCGCT encodes the N-Ala-Gly-Cys-Gly-Cys-Pro-Cys-Gly-Cys-Gly-Ala-C sequence inserted in LamB-153 residue because permissive loop (between structural codons 153and 154) can be exposed to the external medium without a loss of function.


References